Journal: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

Article Title: Identifying patients with bacterial infections using a combination of C-reactive protein, procalcitonin, TRAIL, and IP-10 in the emergency department: a prospective observational cohort study.

doi: 10.1016/j.cmi.2018.09.007

Figure Lengend Snippet: Fig. 2. ROC curves of CRP, PCT, TRAIL and IP10 for suspected and confirmed bacterial infections. AUC, area under curve; CRP, C-reactive protein; IP-10, interferon-gamma induced protein-10; PCT, procalcitonin; ROC curve, receiver operator characteristic curve; TRAIL, tumour necrosis factor-related apoptosis-inducing ligand.

Article Snippet: TRAIL and IP-10 levels were determined using Human TRAIL/ TNFSF10 Quantikine ELISA Kit and Human CXCL10/IP-10 Quantikine ELISA Kit of R&D Systems according to the manufacturer's manual.

Techniques:

Journal: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

Article Title: Identifying patients with bacterial infections using a combination of C-reactive protein, procalcitonin, TRAIL, and IP-10 in the emergency department: a prospective observational cohort study.

doi: 10.1016/j.cmi.2018.09.007

Figure Lengend Snippet: Fig. 3. ROC curves of CRP, PCT, TRAIL and IP10 for suspected and confirmed bacterial infections. AUC, area under curve; CRP, C-reactive protein; IP-10, interferon-gamma induced protein-10; PCT, procalcitonin; ROC curve, receiver operator characteristic curve; TRAIL, tumour necrosis factor-related apoptosis-inducing ligand.

Article Snippet: TRAIL and IP-10 levels were determined using Human TRAIL/ TNFSF10 Quantikine ELISA Kit and Human CXCL10/IP-10 Quantikine ELISA Kit of R&D Systems according to the manufacturer's manual.

Techniques:

Journal: Cancers

Article Title: Targeting of CD133+ Cancer Stem Cells by Mesenchymal Stem Cell Expressing TRAIL Reveals a Prospective Role of Apoptotic Gene Regulation in Non-Small Cell Lung Cancer

doi: 10.3390/cancers11091261

Figure Lengend Snippet: TRAIL sensitivity by in vitro characterization. ( A ) The half maximal inhibitory concentration (IC 50 ) value was determined in non-small cell lung cancer (NSCLC) cell lines (H460, H2170 and A549) by MTS assay after 48 h of rhTRAIL treatment at different concentrations. ( B ) Analysis of TRAIL receptors expression [agonist receptors (DR4 and DR5) and decoy receptors (DcR1 and DcR2)] in MSCs and NSCLC cell lines by flow cytometry. ( C ) Sensitivity of NSCLC cell lines and MSCs evaluated by MTS assay after rhTRAIL (500 ng/mL) treatment.

Article Snippet: The NSCLC cell lines were analyzed for TRAIL agonist (DR4 and DR5) and other decoy receptors (DcR1 and DcR2) with allophycocyanin (APC)-conjugated TRAIL-R1 (DR4) (cat no: FAB347A; Mouse IgG1), TRAIL-R2 (DR5) (cat no: FAB6311A; Mouse IgG 2B ), TRAIL-R3 (DcR1) (cat no: FAB6302A; Mouse IgG1), and TRAIL-R4 (DcR2) (cat no: FAB633A; Mouse IgG1) (R&D Systems, Minneapolis, MN, USA) using flow cytometry (Becton Dickinson BD).

Techniques: In Vitro, Concentration Assay, MTS Assay, Expressing, Flow Cytometry

Journal: Arthritis and rheumatism

Article Title: Mechanisms and clinical relevance of TRAIL-triggered responses in the synovial fibroblasts of patients with rheumatoid arthritis.

doi: 10.1002/art.30181

Figure Lengend Snippet: Figure 4. Rheumatoid arthritis fibroblast-like synoviocytes (RA FLS) produce low levels of the inflammatory cytokines interleukin-6 (IL-6) and IL-8 when stimulated with TRAIL. RA FLS (n 5) were treated with TRAIL (1 nM) or tumor necrosis factor (TNF; 0.5 nM) for 72 hours, and supernatants were analyzed for IL-6 (A), IL-8 (B), and RANTES (C) secretion by enzyme-linked immunosorbent assay. Cy- tokine levels were analyzed using Wilcoxon’s test. Values are the mean SEM. NS not stimulated.

Article Snippet: Serum levels of TRAIL and OPG were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) (Quantikine human OPG ELISA Kit [R&D Systems] and a human TRAIL ELISA [Diaclone]).

Techniques: Enzyme-linked Immunosorbent Assay